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primary antibody against col1 20121 (Novotec Medical GmbH)

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Novotec Medical GmbH primary antibody against col1 20121
Primary Antibody Against Col1 20121, supplied by Novotec Medical GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/col1+primary+antibody+20121/primary+antibody+against+col1+20121/pm39747561-222-2-10
Average 90 stars, based on 1 article reviews

primary antibody against col1 20121 - by Bioz Stars, 2026-11

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Article Title: The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium
Article Snippet: To perform the non-staining total protein loading control, the membranes were exposed for 1 min to UV light (ChemiDoc XRS + System, Bio-Rad, Hercules, CA, USA) to obtain the normalization image. .. The COL1 primary antibody (1:1000 diluted; RRID: AB_2891017, 20121, Novotec, Lyon, France) was incubated overnight at 4 °C and previously validated to equine endometrium by Rebordão et al. [ ]. .. Then, the membranes were incubated for 1.5 h at room temperature with the secondary antibody horseradish peroxidase (HRP)-conjugated anti-rabbit (1:20,000; RRID: AB_2617138; P0448, DakoCytomation, Carpinteria, CA, USA).

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The effect of progesterone (P4; 10 − 7 M) or 17β-estradiol (E2; 10 − 9 M) on mRNA expression of collagen type 1 alpha chain 1 ( COL1A1) ( A ), collagen type 3 alpha chain 1 ( COL3A1) ( B ), matrix metalloproteases (MMP): MMP-1 ( C ), MMP-2 ( D ), MMP-3 ( E ), MMP-9 ( F ), and MMP-13 ( G ), and tissue inhibitors of matrix metalloproteases (TIMPs): TIMP-1 ( H ) and TIMP-2 ( I ) in equine endometrial fibroblasts. Data were analyzed by Student’s t-test and expressed as mean ± SEM. Asterisks designate statistical differences between treatments (* p < 0.05; ** p < 0.01; *** p < 0.001; **** P < 0.0001).

Journal: Scientific Reports

Article Title: Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitro

doi: 10.1038/s41598-024-84250-1

Figure Lengend Snippet: The effect of progesterone (P4; 10 − 7 M) or 17β-estradiol (E2; 10 − 9 M) on mRNA expression of collagen type 1 alpha chain 1 ( COL1A1) ( A ), collagen type 3 alpha chain 1 ( COL3A1) ( B ), matrix metalloproteases (MMP): MMP-1 ( C ), MMP-2 ( D ), MMP-3 ( E ), MMP-9 ( F ), and MMP-13 ( G ), and tissue inhibitors of matrix metalloproteases (TIMPs): TIMP-1 ( H ) and TIMP-2 ( I ) in equine endometrial fibroblasts. Data were analyzed by Student’s t-test and expressed as mean ± SEM. Asterisks designate statistical differences between treatments (* p < 0.05; ** p < 0.01; *** p < 0.001; **** P < 0.0001).

Article Snippet: The primary antibody against COL1 (1:1000 diluted; RRID: AB_2891017, 20121, Novotec, Lyon, France) was incubated overnight at 4ºC, and the secondary antibody horseradish peroxidase (HRP)-conjugated anti-rabbit (1:20,000; RRID: AB_2617138; P0448, DakoCytomation, Carpinteria, CA, USA) was incubated during 1.5 h at room temperature.

Techniques: Expressing

Relative collagen type I alpha chain 2 ( COL1A2 ) mRNA expression of equine endometrial explants obtained from mares in the follicular phase (FP; n = 5) and treated for 24 ( A ) or 48 h ( B ) with medium alone (control), transforming growth factor β1 (TGF-β1; 10 ng/mL), TGF-β1 (10ng/mL) + 17β-estradiol (E2; 10 − 9 M) or E2 alone (10 − 9 M). Results were analyzed by one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparisons test and considered significant at p < 0.05 and shown as mean ± SEM. Asterisks represent significant differences relative to the respective control and asterisks above lines designate differences between treatments (* p < 0.05; ** p < 0.01; **** P < 0.0001).

Journal: Scientific Reports

Article Title: Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitro

doi: 10.1038/s41598-024-84250-1

Figure Lengend Snippet: Relative collagen type I alpha chain 2 ( COL1A2 ) mRNA expression of equine endometrial explants obtained from mares in the follicular phase (FP; n = 5) and treated for 24 ( A ) or 48 h ( B ) with medium alone (control), transforming growth factor β1 (TGF-β1; 10 ng/mL), TGF-β1 (10ng/mL) + 17β-estradiol (E2; 10 − 9 M) or E2 alone (10 − 9 M). Results were analyzed by one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparisons test and considered significant at p < 0.05 and shown as mean ± SEM. Asterisks represent significant differences relative to the respective control and asterisks above lines designate differences between treatments (* p < 0.05; ** p < 0.01; **** P < 0.0001).

Techniques: Expressing, Control

Relative collagen type I (COL1) protein abundance in equine endometrial explants obtained from mares in the follicular phase (FP; n = 5) and treated for 24 ( A ) or 48 h ( B ) with medium alone (control), transforming growth factor β1 (TGF-β1; 10 ng/mL), TGF-β1 (10ng/mL) + 17β-estradiol (E2; 10 − 9 M) or E2 (10 − 9 M), respectively. Results were analyzed by one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparisons test and considered significant at p < 0.05 and shown as mean ± SEM. Asterisks represent significant differences relative to the respective control and asterisks above lines designate differences between treatments (* p < 0.05; ** p < 0.01; *** P < 0.001).

Journal: Scientific Reports

Article Title: Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitro

doi: 10.1038/s41598-024-84250-1

Figure Lengend Snippet: Relative collagen type I (COL1) protein abundance in equine endometrial explants obtained from mares in the follicular phase (FP; n = 5) and treated for 24 ( A ) or 48 h ( B ) with medium alone (control), transforming growth factor β1 (TGF-β1; 10 ng/mL), TGF-β1 (10ng/mL) + 17β-estradiol (E2; 10 − 9 M) or E2 (10 − 9 M), respectively. Results were analyzed by one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparisons test and considered significant at p < 0.05 and shown as mean ± SEM. Asterisks represent significant differences relative to the respective control and asterisks above lines designate differences between treatments (* p < 0.05; ** p < 0.01; *** P < 0.001).

Techniques: Control

Relative collagen type I alpha chain 2 ( COL1A2 ) mRNA expression in equine endometrial explants obtained from mares in the mid-luteal phase (MLP; n = 5), and treated for 24 ( A ) or 48 h ( B ) with medium alone (control), transforming growth factor β1 (TGF-β1; 10 ng/mL), TGF-β1 (10ng/mL) + progesterone (P4; 10 − 7 M) or P4 alone (10 − 7 M). Results were analyzed by one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparisons test and considered significant at p < 0.05 and shown as mean ± SEM. Asterisks represent significant differences relative to the respective control and asterisks above lines designate differences between treatments (* p < 0.05; ** p < 0.01; *** P < 0.001).

Journal: Scientific Reports

Article Title: Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitro

doi: 10.1038/s41598-024-84250-1

Figure Lengend Snippet: Relative collagen type I alpha chain 2 ( COL1A2 ) mRNA expression in equine endometrial explants obtained from mares in the mid-luteal phase (MLP; n = 5), and treated for 24 ( A ) or 48 h ( B ) with medium alone (control), transforming growth factor β1 (TGF-β1; 10 ng/mL), TGF-β1 (10ng/mL) + progesterone (P4; 10 − 7 M) or P4 alone (10 − 7 M). Results were analyzed by one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparisons test and considered significant at p < 0.05 and shown as mean ± SEM. Asterisks represent significant differences relative to the respective control and asterisks above lines designate differences between treatments (* p < 0.05; ** p < 0.01; *** P < 0.001).

Techniques: Expressing, Control

Relative collagen type I (COL1) protein abundance in equine endometrial explants obtained from mares in the mid-luteal phase (MLP; n = 5) and treated for 24 ( A ) or 48 h ( B ) with medium alone (control), transforming growth factor β1 (TGF-β1; 10 ng/mL), TGF-β1 (10ng/mL) + progesterone (P4; 10 − 7 M) or P4 (10 − 7 M), respectively. Results were analyzed by one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparisons test and considered significant at p < 0.05 and shown as mean ± SEM. Asterisks represent significant differences relative to the respective control and asterisks above lines designate differences between treatments (* p < 0.05; *** P < 0.001).

Journal: Scientific Reports

Article Title: Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitro

doi: 10.1038/s41598-024-84250-1

Figure Lengend Snippet: Relative collagen type I (COL1) protein abundance in equine endometrial explants obtained from mares in the mid-luteal phase (MLP; n = 5) and treated for 24 ( A ) or 48 h ( B ) with medium alone (control), transforming growth factor β1 (TGF-β1; 10 ng/mL), TGF-β1 (10ng/mL) + progesterone (P4; 10 − 7 M) or P4 (10 − 7 M), respectively. Results were analyzed by one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparisons test and considered significant at p < 0.05 and shown as mean ± SEM. Asterisks represent significant differences relative to the respective control and asterisks above lines designate differences between treatments (* p < 0.05; *** P < 0.001).

Techniques: Control

Levels of significance ( p values) for two- and three-way interactions between estrous cycle phases, treatment time, and cathepsin G (CAT) or noscapine (NOSC) treatments in the analyses of relative transcript COL1A2 gene and COL1 protein relative abundance. The results were considered significant at p < 0.05.

Journal: Life

Article Title: The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium

doi: 10.3390/life11101107

Figure Lengend Snippet: Levels of significance ( p values) for two- and three-way interactions between estrous cycle phases, treatment time, and cathepsin G (CAT) or noscapine (NOSC) treatments in the analyses of relative transcript COL1A2 gene and COL1 protein relative abundance. The results were considered significant at p < 0.05.

Article Snippet: The COL1 primary antibody (1:1000 diluted; RRID: AB_2891017, 20121, Novotec, Lyon, France) was incubated overnight at 4 °C and previously validated to equine endometrium by Rebordão et al. [ ].

Techniques: Isolation

Inhibition of cathepsin G (0.1 or 1 µg/mL) by noscapine (NOSC; 45 µg/mL) on relative collagen type I alpha 2 chain ( COL1A2 ) mRNA transcription and collagen type I (COL1) protein relative abundance in mare endometrial explants, regardless of estrous cycle phase and time of treatment. Results were considered significant at p < 0.05 and are displayed as least square means ± SEM. Different superscript letters indicate significant differences between CAT concentrations (a, b: CAT 0.1 µg/mL ≠ CAT 1 µg/mL, p < 0.05; c, d: CAT 0.1 µg/mL + NOSC ≠ CAT 1 µg/mL + NOSC, p < 0.05). Asterisks alone represent significant differences relative to the respective control and asterisks above the connecting lines indicate significant differences between treatments (** p < 0.01; *** p < 0.001).

Journal: Life

Article Title: The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium

doi: 10.3390/life11101107

Figure Lengend Snippet: Inhibition of cathepsin G (0.1 or 1 µg/mL) by noscapine (NOSC; 45 µg/mL) on relative collagen type I alpha 2 chain ( COL1A2 ) mRNA transcription and collagen type I (COL1) protein relative abundance in mare endometrial explants, regardless of estrous cycle phase and time of treatment. Results were considered significant at p < 0.05 and are displayed as least square means ± SEM. Different superscript letters indicate significant differences between CAT concentrations (a, b: CAT 0.1 µg/mL ≠ CAT 1 µg/mL, p < 0.05; c, d: CAT 0.1 µg/mL + NOSC ≠ CAT 1 µg/mL + NOSC, p < 0.05). Asterisks alone represent significant differences relative to the respective control and asterisks above the connecting lines indicate significant differences between treatments (** p < 0.01; *** p < 0.001).

Techniques: Inhibition, Control

Effect of cathepsin G (CAT; 0.1 or 1 µg/mL), noscapine (NOSC; 45 µg/mL), or CAT (0.1 or 1 µg/mL) + NOSC (45 µg/mL) treatments in explants of mare endometrium from follicular phase (FP) or mid-luteal phase (MLP) for 24 or 48 h on relative collagen type I alpha 2 chain ( COL1A2 ) mRNA transcription ( A , B ) and collagen type I (COL1) protein relative abundance ( C , D ). Results were considered significant at p < 0.05 and shown as least square means ± SEM. Asterisks alone represent significant differences relative to the respective control and asterisks above connecting lines indicate significant differences of CAT + NOSC treatment relative to the respective CAT-treated group (* p < 0.05; ** p < 0.01; *** p < 0.001).

Journal: Life

Article Title: The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium

doi: 10.3390/life11101107

Figure Lengend Snippet: Effect of cathepsin G (CAT; 0.1 or 1 µg/mL), noscapine (NOSC; 45 µg/mL), or CAT (0.1 or 1 µg/mL) + NOSC (45 µg/mL) treatments in explants of mare endometrium from follicular phase (FP) or mid-luteal phase (MLP) for 24 or 48 h on relative collagen type I alpha 2 chain ( COL1A2 ) mRNA transcription ( A , B ) and collagen type I (COL1) protein relative abundance ( C , D ). Results were considered significant at p < 0.05 and shown as least square means ± SEM. Asterisks alone represent significant differences relative to the respective control and asterisks above connecting lines indicate significant differences of CAT + NOSC treatment relative to the respective CAT-treated group (* p < 0.05; ** p < 0.01; *** p < 0.001).

Techniques: Control

Effect of cathepsin G (CAT; 0.1 or 1 μg/mL), noscapine (NOSC; 45 μg/mL), or CAT (0.1 or 1 μg/mL) + NOSC (45 μg/mL) treatments on relative collagen type I alpha 2 chain ( COL1A2 ) mRNA transcription ( A ) and collagen type I (COL1) protein relative abundance ( B ) in equine endometrial explants treated for 24 or 48 h, regardless of estrous cycle phase. Results are shown as least square means ± SEM and considered significant at p < 0.05. Asterisks above connecting lines indicate significant differences of the same treatment between time of treatment or differences between different concentrations of CAT at the same time of treatment (* p < 0.05; ** p < 0.01).

Journal: Life

Article Title: The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium

doi: 10.3390/life11101107

Figure Lengend Snippet: Effect of cathepsin G (CAT; 0.1 or 1 μg/mL), noscapine (NOSC; 45 μg/mL), or CAT (0.1 or 1 μg/mL) + NOSC (45 μg/mL) treatments on relative collagen type I alpha 2 chain ( COL1A2 ) mRNA transcription ( A ) and collagen type I (COL1) protein relative abundance ( B ) in equine endometrial explants treated for 24 or 48 h, regardless of estrous cycle phase. Results are shown as least square means ± SEM and considered significant at p < 0.05. Asterisks above connecting lines indicate significant differences of the same treatment between time of treatment or differences between different concentrations of CAT at the same time of treatment (* p < 0.05; ** p < 0.01).

Techniques:

Journal: Life

Article Title: The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium

doi: 10.3390/life11101107

Figure Lengend Snippet: Effect of cathepsin G (CAT; 0.1 or 1 μg/mL), noscapine (NOSC; 45 μg/mL), or CAT (0.1 or 1 μg/mL) + NOSC (45 μg/mL) treatments on relative collagen type I alpha 2 chain ( COL1A2 ) mRNA transcription ( A ) and collagen type I (COL1) protein relative abundance ( B ) in explants of mare endometrium from follicular (FP) or mid-luteal (MLP) phases, regardless of treatment time. Results are shown at least square means ± SEM and considered significant at p < 0.05. Asterisks above connecting lines indicate significant differences of the same treatment between estrous cycle phase or differences between different concentrations of CAT at the same estrous cycle phase (* p < 0.05; ** p < 0.01; *** p < 0.001).

Techniques:

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